Bio-Rad CHEF Mapper® XA System - Manual (Page 21)

Screen 2:
Ratio: The ratio between the forward and reverse switch times.
Volts: The actual voltage of the run.
Ramp a: The ramping factor.
To move between the two screens during a run, use the ⇑ or ⇓ cursor keys.
4.4 The Two State Mode
Entering Run Parameters Into Two State
The two state mode allows the separation and resolution of almost all DNA size ranges.
The term two state refers to two field vectors. The directions of these two vectors are deter-
mined by the included angle. This angle is determined relative to the gel, as shown in Figure
4.1. In the two state mode the angle can be continuously varied between 0 and 180°. Linear
or nonlinear switch time ramps are also possible in this mode.
Flg. 4.1. Orientation of the included angle in the two state mode.
TWO STATE prompts the entry of voltage gradient, run time, included angle, and switch
time (ramp or no ramp). When TWO STATE is pushed, the following screen is displayed:
V/cm: Enter the voltage gradient using the number key pad, and press ENTER. The total
voltage applied across the electrodes is obtained by multiplying the gradient by 33.5
Gradient = [ ] V/cm Run Time = [ ]
]
State 1 State 2
Included
angle
➤ ➤
Ratio: # : # ______Volts Ramp a = ________
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