Bio-Rad CHEF Mapper® XA System - Manual (Page 3)

Table of Contents
Page
Section 1 Introduction ..................................................................................................1
1.1 Overview ....................................................................................................................1
1.2
CHEF Mapper System Specifications .......................................................................1
1.3 Description of Major Components ............................................................................3
1.4 Safety ..........................................................................................................................5
Section 2 Operation ......................................................................................................5
2.1 Set-up..........................................................................................................................5
2.2 Operation ....................................................................................................................7
Section 3 Tutorial on the CHEF Mapper System .....................................................7
3.1 Auto Algorithm ..........................................................................................................8
3.2 180° FIGE...................................................................................................................8
3.3 Two State....................................................................................................................9
3.4 Multi State ..................................................................................................................9
3.5 Storing and Recalling a Program in Memory..........................................................10
Section 4 The CHEF Mapper User Interface Display............................................10
4.1 Definitions ................................................................................................................10
4.2 Front
Panel................................................................................................................11
4.3 The FIGE Mode .......................................................................................................14
4.4 The Two State Mode................................................................................................17
4.5 The Multi State Mode ..............................................................................................19
4.6 Clock Read and Delay Start.....................................................................................27
4.7 Storage and Recall of Programs...............................................................................28
4.8 Editing Parameters ...................................................................................................29
Section 5 The Auto Algorithm Mode.......................................................................31
5.1 Operation ..................................................................................................................31
5.2 Applications..............................................................................................................32
Section 6 The Interactive Algorithm .......................................................................33
6.1 Introduction ..............................................................................................................33
6.2
Hardware and Software Requirements ....................................................................34
6.3 Operation ..................................................................................................................34
Section 7 Sample Preparation ...................................................................................38
7.1 Agarose Blocks and Liquid Samples.......................................................................38
7.2 Procedure for S. cerevisiae.......................................................................................38
7.3 Procedure for Mammalian DNA .............................................................................39
Section 8 Gel Electrophoresis....................................................................................44
8.1 Casting the Gel .........................................................................................................44
8.2 Buffer Circulation and Temperature........................................................................45
8.3 Sample Loading........................................................................................................46
8.4 DNA Size Standards ................................................................................................46
8.5 Electrophoresis .........................................................................................................46
8.6
Separations at Room Temperature...........................................................................47
8.7 Removing and Staining the Gel ...............................................................................47