Bio-Rad CHEF Mapper® XA System - Manual (Page 4)

Section 9 Applications ................................................................................................47
9.1 Strategies for Electrophoretic Separations...............................................................47
9.2 Ramping Factor ........................................................................................................49
9.3 Multi State Interrupts ...............................................................................................52
9.4 Pulsed Field Conditions by DNA Size ....................................................................52
9.5 Pulsed Field Conditions by Organism .....................................................................53
9.6 Blotting Megabase DNAs ........................................................................................53
Section 10 Maintenance of Equipment.......................................................................56
10.1 Replacing Electrodes................................................................................................56
10.2 Fuses .........................................................................................................................56
Section 11 Troubleshooting Guide ..............................................................................57
Section 12 References ...................................................................................................59
12.1 Applications in Pulsed Field Electrophoresis..........................................................59
12.2 Reference List for Pulsed Field Electrophoresis .....................................................60
Section 13 Product Information..................................................................................68
Copyright, Bio-Rad Laboratories
All Rights Reserved
CHEF technology is licensed to Bio-Rad Laboratories
Safety
The CHEF Mapper system uses high voltage and current and should be operated with
care at all times. The safety interlocks are for your protection and should not be circumvent-
ed. To avoid shock, set up the CHEF Mapper components in a dry area. Immediately wipe up
any spilled buffers or salt solutions.
When pausing or aborting a run, always check that the current display goes to zero or
displays OFF. This can take 2–5 seconds while the power supply discharges. It is then safe to
remove the lid from the chamber.
Warning: There are high voltages and currents within the chamber, which can be harm-
ful. Do not attempt to circumvent these safety interlocks. Always turn off the power to the
chamber before working within the gel box.
The Cooling Module is ground isolated. Although there is virtually no current flowing
through the Tygon ® tubing into the chiller, you should avoid assembling or disassembling
the tubing while the CHEF Mapper system is operating during a run.
This instrument intended for laboratory use only.
The CHEF Mapper system conforms to the “Class A” standards for electromagnetic
emissions, intended for laboratory equipment applications. It is possible that emissions from
this product may interfere with some sensitive appliances when placed nearby or on the same
circuit as those appliances. The user should be aware of this potential and take appropriate
measures to avoid interference.