Bio-Rad Experion Protein Analysis Kits - Manual (Page 19)

Fig. 1.9. Electropherogram showing the separation of the 1,000 ng/µl sample under nonreducing
conditions.
5. Peaks identified by Experion software are automatically labeled with numbers for easy
identification, and the upper and lower markers used for normalization are indicated in
green with an asterisk (*). To change the type of label displayed, select Graph > Peak
Info or use the drop-down menu and select among the following options:
Peak number –– uses sequential numbers for peak identification (default selection)
Peak time –– uses peak migration time (min:sec) for the peak labels
Peak height –– uses peak height in units of fluorescence intensity for peak labels
Peak corrected area –– uses calculated (corrected) peak area for peak labels
Molecular weight –– uses calculated molecular weight (kD) for peak labels
Peak concentration –– uses calculated concentration (ng/µl) for peak labels
6. Evaluate the data analysis performed by Experion software. Click on the Results tab
and click on any sample in the virtual gel to view the data for that sample.
– To evaluate sizing, examine the values provided in the Mol. Wt. (kD) column for each
sample. BGG is peak 4 in the electropherogram in Figure 1.9 (well 3), and the peak
size is ~158.26 kD.
– To evaluate relative quantitation of the BGG peak, examine the values provided in
the Concentration (ng/µl) column for each sample.
7. Evaluate the reproducibility of the sizing calculation using the coefficient of variation
(%CV), which should be £10%. Click on the Protein Search Criteria tab and click on
the Add Protein Name icon . Replace Change Protein Name 1 with BGG in the
Protein Name column, enter 160 in the Mol Wt. column, and enter 16 in the ± kDa
column.
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