Bio-Rad Experion Protein Analysis Kits - Manual (Page 21)

10. The relative concentration of the BGG, as determined against the known concentration
of the upper marker, is shown in the Concentration (ng/µl) column. To perform an
evaluation of quantitation reproducibility, calculate the %CV, which is defined as the
standard deviation/mean x 100. To do this, export the data to a Microsoft Excel or other
spreadsheet application either by entering the values manually in Excel or by using the
data export feature of Experion software.
11. To export the Protein Table data:
Click the Protein Search Results tab. Right-click on the table, select Copy Table(s) >
Selected Table to Clipboard, and paste into an Excel spreadsheet. Delete the three
lines of calculation at the bottom.
-Or-
Select File > Export Data… The Export window opens. Deselect Result Table, and
select Protein Table. Click Export.
In the Save As window, name the file and designate a folder for it. Click Save.
Open the file in Excel software. Delete the three lines of calculation at the bottom.
12. In the Excel spreadsheet, sort the data by protein concentration. Insert a column
between columns D and E, and label it Exp Conc (Expected Concentration). Enter the
expected concentration for each well (enter 250 for wells 1, 4, 7, and 10; 500 for wells
2, 5, and 8; 1,000 for wells 3, 6, and 9). Do not enter units, as these will interfere with
the sort function. Place the cursor on column E (Exp Conc) and select Data > Sort.
13. Insert 3 columns after column G, and label them Mean Conc, Stdev, and %CV. Use
Excel software to determine the mean, standard deviation, and the %CV (standard
deviation/mean x 100) for each set of replicates (each concentration examined). The
%CV for this test should be £20%.
Expected Performance of BGG in Test 1
Measurement Typical Results
Sizing
Reproducibility %CV £ 10%
Accuracy 160 ± 16 kD
Relative quantitation
Reproducibility %CV £ 20%
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