Bio-Rad Experion Protein Analysis Kits - Manual (Page 7)

Additional Materials and Equipment Required
• Experion automated electrophoresis station
• Experion priming station
• Microcentrifuge (1,000–10,000 x g)
• Aluminum foil
• Heating block or water bath set at 95–100°C
• Benchtop vortexer
• Extra spin filter for additional filtration steps (optional, catalog #700-7254)
• Ice
The Experion Pro260 Starter Kit Tests
The Experion Pro260 starter kit includes all the reagents, consumables, and instructions
required to perform three tests, each of which demonstrates a key protein analysis function
of the Experion system. Each test uses various dilutions and preparations of a standard
protein, bovine g-globulin (BGG), and each test follows the standard Experion Pro260
assay workflow, which is summarized below.
Experion Pro260 Starter Kit Tests
Test Name Purpose Description
1 Sizing and Used for familiarization with the BGG samples are analyzed using
relative Experion system and for standard nonreducing conditions
quantitation troubleshooting. Demonstrates and are quantitated using relative
the basic functions of a Pro260 quantitation against the upper
assay and how to perform relative marker
quantitation, the easiest, fastest
method of protein concentration
determination. Also demonstrates
protein sizing and how to
determine the accuracy and
reproducibility of a Pro260 analysis
2 Percentage Used for demonstration of the BGG samples are analyzed under
determination percentage determination function reducing conditions to allow
of Experion software, which can be separation of the BGG subunits. The
used for rapid evaluation of sample heavy- and light-chain fragments are
purity used to demonstrate the percentage
determination calculations
3 Absolute Used for demonstration of the Five BGG samples at various
quantitation absolute quantitation method, which concentrations are used to generate
using a involves creation of a calibration the calibration curve used for
calibration curve. Demonstrates how to quantitation of another BGG sample
curve generate and evaluate the linearity
of a calibration curve and how to
determine the accuracy and
reproducibility of absolute
quantitation. The results
demonstrate how use of a
calibration curve can
significantly improve
quantitation accuracy
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