Bio-Rad Experion Protein Analysis Kits - Manual (Page 6)

The Experion Pro260 starter kit was designed to introduce new users to protein analysis
with the Experion system as well as for performing troubleshooting, performance
maintenance, or performance qualification of the Experion system. The starter kit is
derived from the Experion Pro260 analysis kit, which is used with the Experion system to
perform separation and analysis of proteins between 10–260 kD under denaturing conditions.
The starter kit includes the Experion Pro260 ladder, which contains nine highly purified
recombinant proteins with molecular masses from 10 to 260 kD and has been optimized
for automated electrophoresis on the Experion system. The kit also features the Pro260
sample buffer, which contains a lower marker and an upper marker used for the proper
alignment of samples to the Pro260 ladder. The starter kit contains all of the components
of the Experion Pro260 analysis kit in addition to other supplies that allow a new user to
start using the system immediately.
For more information on protein analysis using the Experion system, refer to Appendix A of
this manual.
Kit Components and Storage Conditions
Experion Pro260 Starter Kit Components and Storage Conditions
Component Storage Conditions
Experion Pro260 chips, 3 Ambient
Reagents for analysis 4°C
Pro260 gel, 2 vials, 520 µl each
Pro260 ladder, 1 vial, 60 µl
Pro260 sample buffer, 400 µl
Pro260 stain, 45 µl
Spin filters, 2
Bovine g-globulin (BGG) standard (2.0 mg/ml), 2 ml 4°C
Dithiothreitol (DTT) 4°C
DEPC-treated 1 water, 50 ml Ambient
Experion electrode cleaner, 25 ml Ambient
Experion cleaning chip, 1 Ambient
Foam swabs, 25 Ambient
Narrow-bore 20 µl pipet tips, 96 (1 rack) Ambient
0.65 ml RNase-free microcentrifuge tubes, 50 Ambient
Experion Training Video: Chip Loading (DVD) Ambient
Instructions Ambient
1 DEPC treatment of water is not required for the Pro260 assay. Use ultrapure (at minimum 0.2 µm-filtered) water. Do not use
autoclaved water.
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