Bio-Rad Experion Protein Analysis Kits User Manual
Page 25
Fig. 2.3. Separation of the 1,000 ng/µl sample under reducing conditions. Under reducing conditions,
BGG separates into light- and heavy-chain fragments.
4.
Evaluate the percentage determination by Experion software (% Total calculation). Click
on the Results tab, click on lane 2 in the virtual gel, and evaluate the values provided
in the % Total column for each peak. For the sample shown in Figures 2.3 and 2.4, the
light- and heavy-chain peaks correspond to peaks 6 and 8 and represent 34% and
62% of the total protein concentration, respectively.
Fig. 2.4. Results table from Test 2 showing % Total (percentage determination) calculations.
Results are shown for each of the peaks in the electropherogram shown in Figure 2.3.
21
10010510A.qxp 2/13/2008 11:43 AM Page 21