Bio-Rad Bio-Plex Pro™ RBM Canine Kidney Toxicity Assays - Manual (Page 15)

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Volume of Volume of
Panel Sample Dilution Urine Sample, µl Sample Buffer, µl
Human Tox 1 1:4 20 60
Human Tox 2 1:50 10 490
Rat Tox 1 1:2 40 40
Rat Tox 2 1:50 10 490
Rat Albumin 1:10,000 5 (A. Prepare 1:100) 495
5 (B. Prepare 1:100) 495
Canine Tox 1 1:15 10 140
Canine Albumin 1:10,000 5 (C. Prepare 1:100) 495
5 (D. Prepare 1:100) 495
Note: Controls are ready to use after reconstitution. No dilution is needed.
5. Prepare Samples
General guidelines for preparing urine samples are provided here. For more
information contact Bio-Rad Technical Support.
1. Centrifuge urine samples at 500 x g for 5 min to remove particulates.
2. Transfer the cleared samples into new aliquot tubes.
3. Place on ice for immediate use, or store as single-use aliquots at –70 ˚C.
Avoid multiple freeze-thaws.
4. Thaw frozen samples completely prior to dilution.
5. Within 1 hr after thawing, prepare sample dilutions in polypropylene tubes
(such as Titertube ® micro test tubes) as recommended in Table 5. Dilution
scenarios provided are sufficient to run each sample in duplicate.
6. Equilibrate diluted samples to room temperature just prior to the start of
the assay. Do not freeze diluted samples.
Table 5. Summary of recommended sample dilutions.