Bio-Rad Bio-Plex Pro™ RBM Canine Kidney Toxicity Assays - Manual (Page 22)

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Acquire Data
1. Shake the assay plate at 850 ± 50 rpm for 30 sec, and visually
inspect the plate to ensure that the assay wells are filled with buffer.
Slowly remove the plate seal and any plate cover before placing
the plate on the plate carrier.
2. Click Run Protocol and on the pop-up screen, select Load Plate
and click OK to start acquiring data.
3. Use the Wash Between Plates command after every plate run
to reduce the possibility of clogging the instrument.
4. If acquiring data from more than one plate, empty the waste bottle
and refill the sheath bottle after each plate (if HTF are not present).
Select
Wash Between Plates and follow the instructions. Then repeat
the Prepare Protocol and Acquire Data instructions.
5. When data acquisition is complete, select Shut Down and
follow the instructions.
Data Analysis
Quality Controls
If the quality controls were run in the assay plate, open the results (.rbx)
file, click on Report Table, and locate the control wells. Compare the
observed concentrations against the lot-specific control ranges in the
product data sheet.
Note: Expected control ranges are provided for reference and should be
used as general guidelines. Actual results may vary for some operators.
If the controls do not fall within the expected ranges, please refer to the
troubleshooting section for possible causes and solutions.