Bio-Rad Bio-Plex Pro™ RBM Canine Kidney Toxicity Assays - Manual (Page 18)

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7. Read Plate
Bio-Plex Manager ™ software is recommended for all Bio-Plex Pro ™ assay
data acquisition and analysis. Instructions for Luminex xPONENT software
are also included. For instructions using other xMAP system software
packages, contact Bio-Rad Technical Support or your regional Bio-Rad
field applications specialist.
Prepare Protocol in Bio-Plex Manager Software
Version 6.0 and Higher
The protocol should be prepared in advance so that the plate is read as
soon as the experiment is complete.
A protocol file specifies the analytes used in the reading, the plate wells
to be read, sample information, the values of standards and controls,
and instrument settings. Protocols may be obtained from within Bio-Plex
Manager software version 6.0 and higher or created from the File menu.
To create a new protocol, select File, then New from the main menu.
Locate and follow the steps under Protocol Settings.
1. Click Describe Protocol and enter information about the assay
(optional).
2. Click Select Analytes and create a new panel. Visually confirm the
selected analytes and proceed to step 3.
a. Click the Add Panel button in the Select Analytes toolbar.
Enter a new panel name. Select Bio-Plex Pro Assay Magnetic
from the assay dropdown menu. If using Bio-Plex Manager version
5.0 or lower, select MagPlex from the assay dropdown list.
b. Click the Add button. Enter the bead region number and name
for the first analyte. Click Add Continue to repeat for each analyte
in the assay.
For reference, bead regions for the individual assays are listed
in Table 8.