Bio-Rad Bio-Plex® Precision Pro™ Human Cytokine Assays - Manual (Page 11)

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Section 7
Control Preparation (Optional)
One tube of lyophilized cytokine control is provided in each Bio-Plex ® Precision
Pro™ cytokine assay. The preparation of high, medium, and low controls is
optional to monitor plate-to-plate variations. This section provides instructions
on how to reconstitute the Iyophilized control. The product insert provided with
the assay lists the concentration of the reconstituted control. The reconstituted
control can then be further diluted to prepare any concentration of user-
specified quality controls. To ensure optimal assay performance, the cytokine
controls should be prepared in a manner consistent as that used to prepare the
cytokine standards.
Reconstitute Cytokine Controls
1. Gently tap the glass vial containing the lyophilized cytokine control on
a solid surface to ensure the pellet is at the bottom.
2. Reconstitute 1 vial of lyophilized control with 500 µl of the appropriate
diluent. Do not use assay buffer to dilute controls. This is identified
as C0 in the product insert provided with the assay.
3. Gently vortex 1 – 3 sec and incubate on ice for 30 min. Be consistent
with the incubation time to ensure optimal assay performance.
4. The reconstituted cytokine control should be further diluted to create
the desired QC samples in the same diluents specified in the table
above. To obtain the concentration of each reconstituted cytokine
control, refer to C0 in the product insert provided with the assay.
Serum Serum standard diluent
Plasma Plasma standard diluent
Culture supernatant Same culture medium used
to prepare samples
Sample Diluent