Bio-Rad Bio-Plex® Precision Pro™ Human Cytokine Assays - Manual (Page 22)

Section 10
Troubleshooting Guides
This troubleshooting guide addresses problems that may be encountered with
Bio-Plex ® Precision Pro™ cytokine assays. If you experience any of the problems
listed below, review the possible causes and solutions provided. This will assist
you in resolving problems directly related to how the assay steps should be
performed. Poor assay performance may also be due to the Bio-Plex array
reader. To eliminate this possibility, we highly recommend use of the Bio-Plex
validation kit. This kit will validate all the key functions of the array reader and
assist the user in determining whether or not the array reader is functioning
properly.
Possible Causes Possible Solutions
20
High Inter-Assay CV
Standards were not
reconstituted consistently Incubate the reconstituted
standards for 30 min on ice. Always
be consistent with the incubation
time and temperature.
Reconstituted standards and
diluted samples were not stored
properly Reconstituted standards and diluted
samples should be prepared on ice
as instructed. Prior to plating, the
reconstituted standards and diluted
samples should be equilibrated to
room temperature.
High Intra-Assay CV
Bottom of filter plate not dry
Dry the bottom of the filter plate with
absorbent paper towel (preferably
lint-free) to prevent cross-
contamination.