Bio-Rad Bio-Plex® Precision Pro™ Human Cytokine Assays - Manual (Page 20)

18
Acquire Data
1. Shake the assay plate at 1,100 rpm for 30 sec immediately before
acquiring data. Failure to do so will result in increased data acquisition
time due to bead settling.
2. Check that the filter plate is flat. While pressing on one end of the
plate, observe the distance that the opposite end of the plate is
raised off a flat surface. If the distance is >1 mm, transfer all contents
to a flat-bottom 96-well plate or another filter plate.
3. Visually inspect the plate and ensure that the assay wells are filled with
buffer prior to placing the plate in the Bio-Plex microplate platform.
4. Slowly remove the sealing tape and any plate cover before placing
the plate in the reader.
5. Select Step 7 (Run Protocol):
a) Specify data acquisition for 100 beads per region.
b) In Advanced Settings, set the Bead Map to 25 region.
NOTE: Bio-Plex Precision Pro cytokine assays contain magnetic
beads and require the use of the 25 region map available in Bio-
Plex Manager software version 4.1 or higher.
c) In Advanced Settings, set the sample size to 50 µl.
d) In Advanced Settings, confirm that the default DD gate values
are set to 5000 (low) and 32000 (high).
NOTE: When using a Luminex instrument, set the gates
according to the Luminex procedure located in the manual.
e) Select Start and save the .rbx file. Then follow the instructions for
data acquisition.