Bio-Rad Bio-Plex® Precision Pro™ Human Cytokine Assays - Manual (Page 21)

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6. If acquiring data from more than one plate, empty the waste bottle
and refill the sheath bottle after each plate (if HTF not present). Select
Wash Between Plates and follow the instructions for fluidics
maintenance. Then repeat the Prepare Protocol and Acquire Data
steps.
NOTE: Use the Wash Between Plates command after every plate
run to reduce the possibility of clogging the instrument.
7. When data acquisition is complete, select Shut Down and
follow the instructions.
Reacquire Data
It is possible to acquire data from a well or plate a second time using the
Rerun/Recovery mode located below Start in Step 7 (Run Protocol).
1. Check the wells where data will be acquired a second time.
Any previous data will be overwritten.
2. Remove the buffer by vacuum filtration and add 125 µl of assay
buffer to each well. Cover the filter plate with a new sheet of
sealing tape.
3. Repeat Acquire Data steps 1–6 to acquire data a second time.
The data acquired should be similar to the data acquired initially;
however, the data acquisition time will be extended since fewer
beads are present in each well.