Bio-Rad Bio-Plex® Precision Pro™ Human Cytokine Assays - Manual (Page 18)

Add the new target value to the Luminex software by selecting
Calibrate, then New under the Reporter Channel in the Start
Calibration dialog. Enter the new target value and save it as a new lot.
Then calibrate using the new RP1 target value.
2. Select OK and follow the instructions for CAL1 and CAL 2 calibration.
Prepare Protocol
1. Open a new protocol by selecting File, then New from the main
menu. Locate the steps at the left of the protocol menu.
NOTE: To minimize data entry, preset lot-specific Bio-Plex Precision
Pro cytokine assay protocols are available for download at
www.bio-rad.com/bio-plex
2. Select Step 1 (Describe Protocol) and enter information about the
assay.
3. Select Step 2 (Select Analytes) and choose the panel for Cytokines.
Choose the target proteins for the assays on the plate. Note that this
information will already be entered with the preset downloaded
protocol.
Plate Formatting Example
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