Bio-Rad Bio-Plex® Precision Pro™ Human Cytokine Assays - Manual (Page 10)

2. Pipet the appropriate volume of standard diluent into the tubes (see
diagram below). Use serum standard diluent for serum samples,
plasma standard diluent for plasma samples, and culture medium for
culture samples.
3. Add 25.6 µl of the reconstituted standard to the first 1.5 ml tube
containing 374.4 µl of standard diluent. Vortex gently. This is
identified as S1 in the diagram below and in the product insert
provided with assay.
4. Continue making serial dilutions of the standard as shown. After
making each dilution, vortex gently and change the pipet tip after
every transfer.
NOTE: Running an additional two 0 pg/ml blanks is strongly
recommended. Use 50 µl of the appropriate standard diluent as the
blank sample. The 0 pg/ml points should be formatted as blanks,
not as points in the curve, when using Bio-Plex Manager software.
The blank wells are also useful for troubleshooting and determining
LOD.
5. Keep the standards on ice until ready for use. Standards should be
used immediately and should not be frozen for future use.
Standard Dilution Series
8