Bio-Rad BioLogic Sample Loading Loops - Manual (Page 12)

6. Some air may still be trapped in the sample chamber of the DynaLoop. If so, switch the
injection valve to the INJECT position and again empty the sample chamber of the
DynaLoop to the column inlet waste container.
7. Continue to purge all the air from the system by switching between LOAD and INJECT
as described in steps 5 and 6. Terminate this purging procedure with the valve in the
LOAD position and with the sliding seal assembly in contact with sample end fitting.
Stop the BioLogic DuoFlow and the EP-1 Econo pump. Reconnect port 4 to the inlet of
the column. The system is now ready for use.
Electrical Connection (Econo Gradient Pump)
1. Turn off the BioLogic DuoFlow controller and workstation.
2. The Econo Gradient Pump (EGP) is connected to the DuoFlow workstation with bus
communication cable (System cable 17, catalog number 750-0650) (Figure 8). For a
complete discussion of the Econo Gradient Pump, refer to its separate instruction manual.
Fig. 8. Connecting the Econo Gradient Pump to the BioLogic DuoFlow workstation using System Cable 17.
Plumbing Connections
1. Use the fittings kit supplied with the Econo gradient pump and refer to the Econo gradient
pump instruction manual for all peristaltic plumbing connections.
2. To connect the outlet end of the peristaltic pump tubing to port 2 on the injection valve,
use a luer to 1/4-28 adaptor (catalog #732-0113).
Purge Lines
1. Disconnect the tubing from the column at the column inlet connection and redirect Port 4
to a waste container.
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