Bio-Rad BioLogic Sample Loading Loops - Manual (Page 18)

Caution: Do not pry against the black plastic lip on the end fitting that holds the O-ring.
The connector tip may break, causing the DynaLoop to leak.
Installing New Filter
1. Check that the new filter contains a distributor and snap the replacement filter onto the
black connector tip.
2. Insert the buffer end cap into the glass column and thread the end cap into the plastic
jacket by turning clockwise.
4.4 Replacing O-Rings
The DynaLoop contains three O-rings; one on each end cap assembly, and a third on
the sliding seal housing. The O-rings on the buffer and sample connectors are identical.
The O-ring on the sliding seal assembly is
PTFE-coated to reduce friction.
End Cap O-Rings
1. To access the end cap O-rings, unscrew the end caps from the plastic shield and
remove them from the glass column.
2. Carefully remove the O-rings from the connector tips. On the buffer end, remove the
filter assembly first (see Section 4.3, Replacing the Filter).
Caution: Do not scratch the connector tip where the O-ring sits. If the connector tip is
scratched, the DynaLoop will leak.
3. Slide a new O-ring over each end cap. Reinstall the filter on the buffer end cap.
Sliding Seal O-Ring
1. With the end caps removed, use a long blunt instrument to push the sliding seal from
inside the glass tube.
2. Unscrew the cap at the O-ring end of the sliding seal housing.
3. Lift off the O-ring and replace it with a new one.
4. Screw the cap into the housing, making sure that the check valve spring enters the hole
in the center of the cap.
5. Insert the sliding seal assembly into the glass tube making sure the O-ring end of the
assembly is toward the sample end of the tube.
Caution: If the sliding seal assembly is inserted backwards, the DynaLoop can
overpressurize and break when reconnected to the system.
6. Insert the sample end cap into the sample end of the tube. (The sample end cap does
not have the filter.)
7. Slide either end of the protective plastic jacket over the glass tube and screw it onto the
sample end cap.
8. Insert the buffer end cap (with the white filter), into the glass column and thread the end
cap into the plastic jacket. Hand tighten the assembly.
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