Bio-Rad BioLogic Sample Loading Loops - Manual (Page 8)

Important Considerations
• Always follow good laboratory practices when handling solvents.
• Use buffers and solvents that are chemically clean and free of particulates; purify and
filter all buffers and solvents as necessary.
• The wetted materials of the DynaLoop are Tefzel, glass-filled Ryton (polyphenylene
sulfide),
PTFE, and PEEK (polyether-ether ketone). The check valve ball and
O-rings, which are made from nitrile rubber (Buna-N), are compatible with alcohols,
aqueous buffers, and dilute aqueous acids and bases, but will swell when exposed to
solvents typically used for HPLC. If in doubt, test for solvent compatibility before using
the DynaLoop. Immerse the components in a beaker of solvent overnight and examine
for stability.
• The external sleeve is made from polycarbonate, which may lose some of its
transparency if exposed to organic solvents or highly acidic or basic fluids.
• Be careful when changing from buffered aqueous solvents to organic solvents. Flush
the system with Milli-Q or HPLC-grade water or another appropriate solvent before
introducing an organic solvent. Otherwise, the buffer salts may precipitate and block the
check valve or other parts of the unit.
• If the DynaLoop is not to be used for several days, remove buffers and store the loop in
a 20% ethanol solution. For longer periods of storage, refer to Section 4.2, Cleaning
and Storage.
• Do not kink, nick, or sharply bend the tubing. Bent or damaged tubing restricts flow and
eventually causes tubing or fitting failure.
2.3 Installation
Overview
This section describes how to connect the DynaLoop to the BioLogic DuoFlow system
with an AVR7-3 automated injection valve.
Auxiliary Pump Loading
An auxiliary peristaltic pump can be programmed to automatically fill the DynaLoop prior
to sample injection.
General Instructions
Before using the DynaLoop for the first time, clean the unit to remove packing dust and
debris. See Section 4.2, Cleaning and Storage, for directions on disassembling, cleaning,
and reassembling the unit.
Preparation
1. Attach the DynaLoop to the BioLogic DuoFlow system. Measure the lengths of tubing
required to make the desired connection. Allow enough slack so that the tubing is not
pulled tightly around sharp corners.
2. Using the tubing cutter supplied with the BioLogic DuoFlow System Fittings kit, cut the
tubing supplied with the DynaLoop to the desired length.
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