1 dynaloop sample loop description, 2 operation – Bio-Rad BioLogic Sample Loading Loops User Manual
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Section 1
Introduction
1.1 DynaLoop Sample Loop Description
The DynaLoop sample loop is a large-volume sample injection loop for use with the
BioLogic DuoFlow
™
system. Available in 25 ml and 90 ml sample capacities, the DynaLoop
is used with the automated AVR7-3 injection valve.
1.2 Operation
The DynaLoop consists of five major components (Figure 1):
•
Sample end assembly
•
Buffer end assembly
•
Glass tube graduated in 1 ml increments
•
Sliding seal assembly
•
Protective plastic jacket
Fig. 1. DynaLoop 25 and DynaLoop 90 sample loops.
The sliding seal assembly divides the glass tube into two distinct chambers:
•
Sample chamber
This end connects to port 3 on the AVR7-3 injection valve.
•
Buffer chamber
This end connects to port 6 on the AVR7-3 injection valve.
The DynaLoop sample loop delivers sample to the column in a four-phase process:
•
Load phase
•
Injection phase
•
Flush phase
•
Reload phase
Sample end
assembly
Sliding seal
assembly
Glass tube
Buffer end
assembly
Plastic jacket
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