Bio-Rad BioLogic Sample Loading Loops - Manual (Page 13)

2. Connect the Econo gradient pump inlet tube to a reservoir of buffer. Switch the injection
valve to the INJECT position. Set the BioLogic DuoFlow flow rate to 10 ml/min. Verify
that the DynaLoop fills with buffer.
Caution: The sliding seal must move toward the sample end of the DynaLoop. If the seal
moves toward the buffer end of the DynaLoop (the end with the white filter), stop the pump
immediately. The DynaLoop was installed backwards. Correct the plumbing configuration
before using the DynaLoop.
3. Manually start the Econo gradient pump. Buffer should flow to the drain tube at port 1.
4. Continue running the BioLogic DuoFlow pump and Econo gradient pump until the
DynaLoop and all tubing associated with the Econo gradient pump are completely
purged of air. Flow into the waste containers should form a smooth stream free of bubbles.
When the sliding seal assembly contacts the sample end cap, the check valve inside
the seal will open allowing air and buffer to escape.
5. With both pumps still running, switch the injection valve to LOAD. Flow from the
peristaltic pump will move the sliding seal towards the buffer end of the DynaLoop.
Flow from the BioLogic DuoFlow pump will go through the injection valve directly to port
4 and then temporarily to waste.
6. Some air may still be trapped in the sample chamber of the DynaLoop. If so, switch the
injection valve to the INJECT position and again empty the sample chamber of the
DynaLoop to the column inlet waste container.
7. Continue to purge all the air from the system by switching between LOAD and INJECT
as described in steps 5 and 6. Terminate this purging procedure with the valve in the
LOAD position and with the sliding seal assembly in contact with sample end fitting.
Stop the BioLogic and the Econo gradient pump. Reconnect port 4 to the inlet of the
column. The system is now ready for use.
Section 3
Using the DynaLoop
This section describes some techniques for using the DynaLoop.
3.1 Injecting Sample Volume
This section describes the procedures for:
• DynaLoop preparation
• Loading sample manually
• Loading sample automatically
Preparation
Purge air from all system components. To purge the DynaLoop quickly, disconnect the
column inlet and set the BioLogic DuoFlow pump to a higher flow rate. Purge the system
with the valve set to INJECT. Remember to stop the pump and reconnect the column after
the system is completely purged.
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