Bio-Rad Model EG-1 Econo™ Gradient Monitor - Manual (Page 14)

Model EG-1 Econo Gradient Monitor Control Unit and Flow Cell:
The control unit may be left on continually (power consumption is neg-
ligible).
Although the cases are chemical resistant, spills and splashes may
cause precipitates to form on the component cases. Use a squirt bottle
and soapy water to wash down the outer cases of the instrument.
Flow Cell
Clean the outside surface of the flow cell as described above.
The flow cell should be cleaned periodically with 1 M NaOH at 1
ml/min for 5 min. (SDS, 1 M HCl, 1 M NaOH, EtOH, and acetone will
not harm the Model EG-1 Econo Gradient Monitor flow cell.)
When the flow cell is not in use, disconnect, and clean out dissolved
salts using a syringe filled with deionized water.
Store the flow cell by injecting a dilute solution (15-25%) of ethanol or
isopropanol into the cell to prevent microbial growth. Use the end caps
provided to seal the flow cell’s inlet and outlet ports. Always rinse the
flow cell with water prior to the introduction of aqueous buffers. To
prevent the formation of salt crystals and solute deposits, do not allow
the flow cell to dry out.
Section 8
Troubleshooting
Problem Possible Cause Solution
Baseline drift or Bubbles in flow cell Use degassed buffers.
noisy signal Check for loose plumb-
ing connections
Dirty flow cell Clean flow cell; insure
that buffers are free of
particulate material
(See Section 6)
Bubbles passing Use degassed buffers.
through flow cell Check for loose plumb-
ing connections
Column not Equilibrate and/or
equilibrated or dirty regenerate column
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