Bio-Rad Model EG-1 Econo™ Gradient Monitor - Manual (Page 15)

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Larger or smaller than Chart recorder input Check chart recorder
expected gradient voltage set incorrectly settings (See Sections
profile 4.2 and 5)
If using Gradient Re-calibrate the
mode, buffer A and gradient mode
B values not set
correctly
Ghost peaks appear in Charged sample No problem
gradient profile detected (e.g. protein)
Air bubbles passing Use degassed buffers.
through flow cell Check for loose plumb-
ing connections
Chart recorder Chart recorder is not Check connections (See
not responding connected properly Sections 3.2 and 4.2)
Full scale of recorder Set recorder range to
set too high 1 V
Recorder has bottomed Readjust pen position
out
Flow cell not Check flow cell
connected in-line plumbing
No power Faulty power Check power cable
connection connection
Blown fuse Check fuses (See
Appendix A)
Unit not switched on Check power on/off
switch on rear panel
Conductivity Dirty flow cell Clean flow cell (See
measurements with Section 6)
same buffer appear
to decrease over time
Problem Possible Cause Solution
M7318150H 2/27/1 2:06 PM Page 12