Bio-Rad Profinity IMAC Resins User Manual
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Resin Preparation
Profinity IMAC and Profinity IMAC Ni-charged resins come supplied in a 20%
ethanol solution for resin storage. Before the column can be slurry packed, the
resin storage solution must be replaced with distilled water.
1.
For easy removal of storage solution, transfer an appropriate amount of
resin slurry to a graduated container, such as a Bio-Rad Econo-Pac
®
column (bed volume up to 20 ml).
2.
Apply vacuum to the column for rapid removal of the storage solution.
The storage solution can be removed rapidly by applying a vacuum to the
column.
3.
Wash the column with 3 column volumes of distilled water.
4.
Add enough distilled water to make a 50% slurry.
5.
The resin is now ready to be packed.
Alternatively, if larger volumes of Profinity IMAC or Ni-charged IMAC resins are
being used, simply resuspend the resin in the ethanol solution provided. Proceed
with the method below and during step 6, ensure that all ethanol is removed during
the equilibration step.
Method
1.
Use a column that is 2.5 times longer than the required bed height.
2.
Eliminate air from the column dead spaces.
Fill the column with distilled water to about 10% of its volume. Flush endpieces
with distilled water to ensure that the bottom of the bed support is fully
saturated and free of air bubbles. Allow a few centimeters of distilled water to
remain before closing the outlet valve.
3.
Suspend the 50% v/v Profinity slurry by gently swirling or stirring with a
glass or plastic rod.
4.
Pour slurry into the column.
Carefully pour the slurry down the side of the column using a glass or plastic
rod so as to not introduce air bubbles.
Note: If using Profinity IMAC uncharged resin, proceed with Immobilizing
Metal Ions procedure (Section 6) once slurry packing is accomplished.
5.
Insert the top flow adaptor.
Use care to not introduce any air bubbles. Insert the adaptor at an angle into
the column. Make sure that the exit tubing is open to allow distilled water to
flow out of the top adaptor along with any air.
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