Bio-Rad Profinity IMAC Resins - Manual (Page 37)

33
Part 3: Eluting the His-Tagged Protein
10. Insert micro spin column into new, clean collection vessel.
11. Elute bound proteins with 5 column volumes of elution buffer.
Pipet up and down at least 5 times and incubate for up to 5 min.
The choice of elution buffer will vary depending on the procedure used. For
example, a range of imidazole concentrations (150–500 mM) may be used to
elute bound protein from the Profinity IMAC resin.
Analyze fractions from above steps by A
280
, SDS-PAGE, ELISA, etc.
10001677B.qxd 1/28/2005 12:42 PM Page 36