Bio-Rad Profinity IMAC Resins - Manual (Page 2)

Table of Contents
Section 1 Introduction ..............................................................1
Section 2 Product Description ................................................1
Profinity ™ IMAC Resins and UNOsphere ™ Technology...1
Chemical Interactions ...................................................2
Resin Characteristics....................................................3
Chemical Compatibilities ..............................................4
Section 3 General IMAC Procedures .....................................5
Protein Binding.............................................................5
Washes ........................................................................5
Elution ..........................................................................6
Purification Under Denaturing Conditions .....................6
Purification Under Nondenaturing Conditions ...............7
Imidazole Concentrations .............................................7
Section 4 Column Packing — Medium-Pressure
Columns ....................................................................9
Slurry Packing an IMAC Column...................................9
Recommended Columns..............................................9
Materials ......................................................................9
Resin Preparation .......................................................10
Method ......................................................................10
Section 5 Column Packing — Sample Preparation-Sized
Columns ..................................................................12
Materials ....................................................................12
Resin Preparation .......................................................12
Method ......................................................................12
Section 6 Immobilizing Metal Ions .......................................14
Section 7 Preparation of Clarified E. coli Lysate
Using Nondenaturing Conditions........................15
Materials ....................................................................15
Method ......................................................................16
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