Bio-Rad Profinity IMAC Resins - Manual (Page 17)

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4. Remove storage solution by centrifugation.
Centrifuge at 1,000 x g for 15 sec to pack resin.
5. Wash column with at least 5 column volumes (or ~500 µl) of distilled
water.
Centrifuge at 1,000 x g for 15 sec to pack resin.
6. If using Ni-charged resin, equilibrate the column with at least 5 column
volumes of binding buffer.
The column is now ready for separation.
Note: Proceed to sections for preparation of lysates and column purification
only if using the Ni-charged Profinity IMAC resin.
7. If using uncharged resin, proceed to Section 6, Immobilizing Metal Ions.
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