Labconco Purifier Filtered PCR Enclosures 3970425 - Manual (Page 26)

Chapter 5: Appropriate Applications for Your Filtered PCR Enclosure
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Loading Materials and Equipment

Load only the materials required for the procedure. Do not overload the
enclosure.

Do not obstruct upper air diffuser screen.

Large objects should not be placed close together.

After loading, wait one minute to purge airborne contaminants from the
work area.
Work Techniques

Keep all materials inside the lower air foil, and perform all operations as
far to the rear of the work area as possible.

Segregate all clean and contaminated materials in the work area.
Final Purging

Upon completion of work, the enclosure should be allowed to operate for
two to three minutes undisturbed, to purge airborne contaminants from the
work area before shutting down the blower.

Select the UV time for your PCR protocol, install the UV closure panel and
turn on the UV light to decontaminate the work area as determined by your
Safety Officer.
Unloading Materials and Equipment

All open trays, weigh vessels or containers should be covered before being
removed from the enclosure.
Suitable Applications
Suitable applications for the Purifier Filtered PCR Enclosures are based on
guidelines established by the Institute for Environmental Sciences. Particulates are
filtered by the HEPA filter before air is returned to the room. Biohazardous
materials or other hazardous solids cannot be used with the Purifier Filtered PCR
Enclosure, as air is drawn over the work surface and out of the enclosure. The
Purifier Filtered PCR Enclosure only provides product protection and offers no
personnel protection whatsoever.
Listed below are suitable applications for HEPA filters.
HEPA Filter Applications, Suitability and
Guidelines

Procedures traditionally performed on an open bench where a clean Class 5 air
environment is now required.

The HEPA filtered enclosure provides product protection from particulate
matter. Because air from the enclosure enters the room during operation, this
enclosure should only be used for operations requiring sample protection from
environmental contamination.