Multichannel Systems Roboocyte2 Scripting Manual - Manual (Page 22)

Roboocyte2 Manual
22
The oocyte loop itself has only 4 lines (lines 89, 90, 91 and 105 in the example shown above),
but, before terminating the oocyte loop by the final curly bracket, do not forget to stop the valve
pump to avoid flooding (lines 101, 102 and 103)
2.3 Standard Routines executed before Recording Protocol
Before starting the recording of data, a number of steps and routines have to be started which
usually will not change for different kind of recording protocols.
2.3.1 Moving the Measuring Head into Liquid
The oocyte loop cares for incrementing the well number, the movement of carrier and z-axis have
to be controlled by specific commands.
Before moving the measuring head into liquid, one should switch the waste pump on to
guarantee a constant buffer level relative to the electrodes. This is important to avoid offset
artifacts during oocyte impalement.
Robo2.WastePumpOn(pumpspeed*ratio/100); starts the waste pump.
The waste pump "speed" is defined by the values for pumpspeed and ratio as defined before.
Robo2.MoveToWell(WellIndex); moves the carrier to the respective well.
Robo2.ZMoveToLiquid(); moves the z-axis down into the well.
The default liquid position can be changed in the Settings/Options menu of the Roboocyte
software (default is 2000 µm above the well bottom).