Multichannel Systems Roboocyte2 Scripting Manual - Manual (Page 24)

Roboocyte2 Manual
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If the electrode resistances are within the range (between MIN_RESISTANCE and
MAX_RESISTANCE) the script proceeds; if not (else), the script breaks and continues with the
next oocyte.
Now, after completing electrode offset compensation, oocyte impalement can be started.
2.3.5 Oocyte Impalement
The oocyte impalement is performed by using the command:
Robo2.Impale(MIN_RMP, IMPALEMENT_STEPS, IMPALEMENT_STEP, IMPALEMENT_WAIT)
MIN_RMP, IMPALEMENT_STEPS, IMPALEMENT_STEP and IMPALEMENT_WAIT are predefined
variables (see table in chapter 1.2.3).
The impalement process is started by a movement of the z-axis to the default oocyte impalement depth
(default = 800 µm above the well plate bottom). Then n =
IMPALEMENT_STEPS are performed with a
step size of
∆
z = IMPALEMENT_STEP and a waiting time t = IMPALEMENT_WAIT between
individual steps.
Default values for IMPALEMENT_STEPS and IMPALEMENT_STEP are 8 and 50 µm, respectively.