Multichannel Systems Roboocyte2 Scripting Manual - Manual (Page 26)

Roboocyte2 Manual
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where MIN_INITIAL_LEAKCURRENT and MAX_INITIAL_LEAKCURRENT are predefined variables
with default values -10000 and +200, respectively. If the leak current is within the range the script
continues after line 209; if not, the run continues with the next selected oocyte. Again, commands
for stopping pumps and valves should be included (lines 205 - 207).
2.3.8 Final Leak Current Test with Perfusion
After the successful initial leak current check, i.e. if the oocyte is not completely leaky, the script
continues with the final leak current check. Although you can start this final check directly after
the initial leak current test, we recommend to start the buffer perfusion of the oocyte beforehand
in order to check the viability of the oocyte under perfusion.
Then, after a waiting time of 5 seconds, the final leak current check can be initiated by the
command
Robo2.LeakCurrentCheck(MIN_LEAKCURRENT, MAX_LEAKCURRENT,
LEAKCURRENT_ATTEMPTS, LEAKCURRENT_WAIT)
where MIN_LEAKCURRENT, MAX_LEAKCURRENT, LEAKCURRENT_ATTEMPTS and
LEAKCURRENT_WAIT are predefined variables. Default values for MIN_LEAKCURRENT and
MAX_LEAKCURRENT are -1000 nA and 100 nA, respectively. Default values for
LEAKCURRENT_ATTEMPTS and LEAKCURRENT_WAIT are 3 and 10 seconds, respectively. This
means that the leak current is determined up to 3 times with a waiting time of 10 seconds in
between.
As soon as the leak current is within the given limits, the script continues after line 237. if the cell
is still too leaky after 3 trials, the script continues with moving to the next well.
After successful leak test, the control recording has to be stopped, and the result of final leak test
can be sent into the log window.
Now, after finishing all necessary preparations and tests, the specific recording protocol can be
commenced.