Multichannel Systems Roboocyte2 Scripting Manual - Manual (Page 34)

Roboocyte2 Manual
34
The transfer ports offer you the option to work with larger solution volumes, e.g. for often used
buffers or compounds. Using the transfer ports would mean moving the needle to the transfer
port with the respective movement commands, opening the respective valves with the commands
listed above, and finally switching the Minipulse pump on to aspirate solution from the transfer
port.
4.4 Examples
Scripts that were written for using the Roboflow can be easily modified to work with the Gilson
liquid handler instead. The following examples demonstrate how Roboflow solution exchange
commands correspond to those of the Gilson liquid handler.
1. Opening a Roboflow valve corresponds to moving the Gilson needle into a tube or transfer
port.
2. Turning on the Roboflow valve pump corresponds to starting the Minipulse pump in backward
direction.
4.4.1 Differences between Roboflow and Gilson
1. Instead of using a manifold the Gilson liquid handler connects the different solution reservoirs
and the measuring head with a single tubing. Therefore, an air gap has to be formed between
two different solutions in the tubing to avoid mixing during transport from the probe to
measuring head.
2. When using Roboflow, there is a delay of 2 to 3 s between the solution exchange command in
the script and the arrival of the solution in the well (i.e. at the oocyte) when using pump speed
5000.
When using the Gilson liquid handler, this delay is much larger due to the tubing length between
Gilson probe and measuring head. Depending on tubing length and the selected pump speed of
the Minipulse pump, this delay can be tens of seconds and has to be considered in the design of
the script.
4.4.2 Solution Exchange - Roboflow vs. Gilson
Defining a solution exchange sequence where solution1 runs for 5 seconds, followed by solution 2
for 10 seconds and finally solution 1 for 10 seconds will be realized by the following sequences of
script commands.
The following table shows how Gilson commands correspond to Roboflow commands.
Roboflow Gilson
Robo2.OpenValve(1); Gilson.MoveToTube(1, 1);
Robo2.ValvePumpOn(5000); Gilson.PumpBackward(2600);
Robo2.Wait(5); Robo2.Wait(5);
solution separating air gap
(Gilson only) Gilson.PumpStop();
Gilson.MoveUp();
Gilson.PumpBackward(2600);
Robo2.WaitMilliSec(600);
Gilson.PumpStop();
Robo2.OpenValve(2); Gilson.MoveToTube(1, 2);
Gilson.PumpBackward(2600);
Robo2.Wait(10); Robo2.Wait(10);
solution separating air gap Gilson.PumpStop();
Gilson.MoveUp();