Multichannel Systems Roboocyte2 Scripting Manual - Manual (Page 25)

Appendix
25
If the impalement was not successful, i.e. if the membrane potential U = MIN_RMP was not
reached after n = IMPALEMENT_STEPS steps with the step distance d = IMPALEMENT_STEP the
run is continued with the next oocyte. Before moving to the next oocyte pumps and valves should
be closed (lines 170 - 172). IMPALEMENT_WAIT is the waiting time between individual steps of
the z-axis in seconds.
If impalement was successful, the script continues after line 175. It usually makes sense to wait at
least for 10 seconds after impalement to give the oocyte membrane enough time to recover from
the impalement.
2.3.6 Starting Voltage Clamp Mode
After successful impalement, the recording can be continued under voltage clamp. Before, scaling
of the Control Display axes should be changed to meet the demands of the following Leak
Current Test under voltage clamp.
Robo2.SetHoldingVoltage(clampvoltage); sets the command voltage .
"clampvoltage" is a user defined variable created at the beginning of the script.
Robo2.SetVoltageClamp();sets the amplifier to voltage clamp mode.
2.3.7 Initial Leak Current Test
After starting voltage clamp, a control recording is started in order to perform the initial leak
current check.
The leak current check is started by the command
Robo2.InitialLeakCurrentCheck(MIN_INITIAL_LEAKCURRENT, MAX_INITIAL_LEAKCURRENT)