Multichannel Systems Roboocyte2 Scripting Manual - Manual (Page 35)

Appendix
35
Gilson.PumpBackward(2600);
Robo2.WaitMilliSec(600);
Gilson.PumpStop();
Robo2.OpenValve(1); Gilson.MoveToTube(1, 1);
Gilson.PumpBackward(2600);
Robo2.Wait(10); Robo2.Wait(10);
Same sequence from above listed sequentially.
Roboflow
Robo2.OpenValve(1);
Robo2.ValvePumpOn(5000);
Robo2.Wait(5);
Robo2.OpenValve(2);
Robo2.Wait(10);
Robo2.OpenValve(1);
Robo2.Wait(10);
Gilson
Gilson.MoveToTube(1, 1);
Gilson.PumpBackward(2600);
Robo2.Wait(5);
Gilson.PumpStop();
Gilson.MoveUp();
Gilson.PumpBackward(2600);
Robo2.WaitMilliSec(600);
Gilson.PumpStop();
Gilson.MoveToTube(1, 2);
Gilson.PumpBackward(2600);
Robo2.Wait(10);
Gilson.PumpStop();
Gilson.MoveUp();
Gilson.PumpBackward(2600);
Robo2.WaitMilliSec(600);
Gilson.PumpStop();
Gilson.MoveToTube(1, 1);
Gilson.PumpBackward(2600);
Robo2.Wait(10);
Synchronizing this sequence with a recording is straightforward when using the Roboflow; put
the command Robo2.StartRecording(); and Robo2.StopRecording(); before and after the
sequence of commands, respectively.
4.4.3 Handling of the Gilson Delay
When using the Gilson liquid handler, the significant delay between switching to a respective
solution and its arrival at the oocyte has to be considered. There are different ways to include this
delay, but the easiest way is to add an additional wait command Robo2.Wait(delay); directly
before the Robo2.StopRecording(); command. The delay time depends on the used Minipulse
pump speed and the length of the tubing between Gilson needle and measuring head. Therefore,
delay time has to be determined experimentally beforehand.
Usually, buffer solution (solution 1) is already running before starting a solution exchange
protocol. Therefore, the sequence after the Robo2.StartRecording(); command starts with the
first wait command Robo2.Wait(5);.
In addition, the respective transmit commands have to be included in order to send the
appropriate compound and concentration information to the database.
Likewise, ROIs have to be defined in a way that the response of interest will be localized within
the ROI.