4using the gilson liquid handler, 1 movement commands, 2 peristaltic pump commands – Multichannel Systems Roboocyte2 Scripting Manual User Manual

Page 33: 3 transfer port valve commands

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Appendix

33

4

Using the Gilson Liquid Handler

Commands controlling the Gilson liquid handler can be separated in movement commands,
commands controlling the peristaltic pump connected to the Gilson liquid handler, and commands
controlling the magnetic valves of the Gilson's transfer ports. If you are using the liquid handler,
scripts have to be modified, using Gilson commands instead of Roboflow commands.

Important note: Racks (and corresponding slots) used in a specific script have to be defined and
selected by means of the Liquid Configuration feature of the Roboocyte software. Otherwise the
script will not work.

4.1 Movement Commands

Gilson.

Parameter(s)

Action

MoveToTube(x,y)

int: x = slot

int: y= tube

moves the probe to tube y in slot x (1 .. 5);
the number of available tubes depends on
the type of rack

MoveToPort(x)

int: port number (1 or 2)

moves probe to transfer ports x (1 or 2)

MoveToRinse()

---

moves probe to the rinse station

MoveToDrain()

---

moves probe to the drain station

MoveUp()

---

moves the Gilson probe up

MoveHome()

---

moves all to home position

Reset()

---

resets the Gilson

The "movement commands" move the Gilson probe (needle) in x, y, and z direction either to
tubes, transfer ports, rinse station or drain station or just up (z-axis) or home (x = y = z = 0). The
Reset command can be used to "reset" the machine, e.g. after some kind of malfunction, and is
usually not used in a script.

4.2 Peristaltic Pump Commands

PumpBackward(x) int: x = speed

starts the Gilson peristaltic pump in
backward direction with speed x

PumpForward(x)

int: x = speed

starts the Gilson peristaltic pump in forward
direction with speed x

Gilson.PumpStop()

---

stops the Gilson peristaltic pump

Whenever the Gilson liquid handler is used, the Minipulse peristaltic pump will be used. This
pump will be then controlled by the Roboocyte software with the script commands listed above.

4.3 Transfer Port Valve Commands

Valve1On()

---

opens valve 1 from transfer port

Valve1Off()

---

closes valve 1 from transfer port

Valve2On()

---

opens valve 2 from transfer port

Valve2Off()

---

closes valve 2 from transfer port

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