Multichannel Systems Roboocyte2 Scripting Manual - Manual (Page 7)

7
offset check
Robo2.Impale(MIN_RMP, IMPALEMENT_STEPS, IMPALEMENT_STEP,
IMPALEMENT_WAIT)
MIN_RMP -15 mV minimum membrane potential
IMPALEMENT_STEPS_I 6 number of z axis steps to move
down during impalement of
the I electrode
IMPALEMENT_STEPS_U 2 number of z axis steps to move
down during impalement of
the U electrode
IMPALEMENT_STEPS 8 maximum number of z axis
steps to move down during
impalement
IMPALEMENT_STEP 50 µm step size of impalement step
IMPALEMENT_WAIT 2 sec wait after each z axis step
Robo2.InitialLeakCurrentCheck(MIN_INITIAL_LEAKCURRENT,
MAX_INITIAL_LEAKCURRENT)
MIN_INITIAL_LEAKCURRENT -10000 nA minimum leak current for initial
check
MAX_INITIAL_LEAKCURRENT 200 nA maximum leak current for
initial check
Robo2.LeakCurrentCheck(MIN_LEAKCURRENT, MAX_LEAKCURRENT,
LEAKCURRENT_ATTEMPTS, LEAKCURRENT_WAIT)
MIN_LEAKCURRENT -1000 nA minimum leak current for
second check
MAX_LEAKCURRENT 100 nA maximum leak current for
second check
LEAKCURRENT_WAIT 10 wait after each check [sec]
LEAKCURRENT_ATTEMPTS 3 number of attempts to try leak
current check
Robo2.SetAmplifierCoefficients(AMPLIFIER_GAIN_P, AMPLIFIER_GAIN_I)
AMPLIFIER_GAIN_P 1000 nA/mV proportional coefficient 0 -
6000
AMPLIFIER_GAIN_I 100 1/s integral coefficient 0 - 1000
Robo2.SetSampleRate(SAMPLERATE);
Robo2.SetControlSampleRate(CONTROL_SAMPLERATE);
SAMPLERATE 1000 Hz recording sample rate 1 - 20000
CONTROL_SAMPLERATE 10 Hz control recording sample rate 1
- 20000
Important note: These predefined variables are not active until they are
explicitly used in a script command. E.g., when the SAMPLERATE variable is set to
10000, this sample rate is only used when the script command
Robo2.SetSampleRate(SAMPLERATE) is executed.
Likewise, if the SAMPLERATE parameter is set to 10000, but the script command
is Robo2.SetSampleRate(1000), the sample rate of 1000 is used for all recordings
in the script.
1.2.4 Read-only Variables
These are variables the values of which cannot be changed by the user but are
updated by the system.