Techne PrimeQ - Manual (Page 157)

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3.18 Analysis method: Multi-read
This analysis method averages all or a selected range of values in the stage for a given well. Multi-
read is a useful tool in end-point analysis; end-point fluorescence values can be compared to Cq
values obtained from quantification analysis of the amplification stage and used to aid primer
optimization experiments. Alternatively, Multi-Read allows PrimeQ to be used simply as a plate
reader.
3.18.1 Assay requirements
• Need at least one reporter.
• Need more than one cycle.
• Need a dye defined as PRD for the PRD correction to be enabled.
3.18.2 Setup
• In the Analysis Selection box, highlight the stage name for analysis to be applied and click
Edit. The Analysis Wizard will launch.
• Choose Multi-Read from the drop-down menu in the Analysis Wizard Selection box and
assign dyes usage(s).
• Click Next and the Multi-Read Wizard will launch.
If a PRD was assigned in the Dye Usage Box then the next screen provides the option to apply
PRD correction to the data.
• Click Next.
3.18.2.1 Multi-read selection method
The next screen allows the user to choose which readings are to be averaged.
Raw data for reporter with
or without PRD correction
Average the
selected values
Report Raw data for reporter with
or without PRD correction
Average the
selected values
Report