Techne PrimeQ - Manual (Page 17)

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1.3 Unpacking
When unpacking the unit, check that the following have been removed from the packing:
• This PrimeQ operator guide
• A CD containing an electronic copy of this guide and Quansoft software
• The PrimeQ unit including block
• Filter cartridges in box
• Mains lead(s)
• Guarantee card
• Decontamination certificate
• USB cable
Keep the original packaging in case you ever need to return the unit for service or repair. Techne
accepts no responsibility for damage incurred during transportation unless the unit is correctly
packed and transported in its original packaging.
The instrument weighs 25kg (55lb). Take care when lifting – this is best done by two people.
1.4 Warning!
Position the unit so that the mains on/off switch is accessible. If a safety problem
should be encountered, switch off at the power socket and remove the plug from
the supply.
If you are using more than one unit in close proximity to each other, there must be
at least 100mm between the units to allow the cooling air to flow correctly.
1.5 Uses of PrimeQ
PrimeQ has many scientific laboratory applications. Aspects of the PCR process are claimed in
U.S. Patent Nos. 5,475,610 (claims 160-163 and 167) and 6,703,236 (claims 1-6) or
corresponding claims in their non-US counterparts. Use of PrimeQ in such processes is covered
by licenses granted to Techne by Applied Biosystems LLC (except for use in human in vitro
diagnostics).
1.6 Plates
Techne recommends only the type of low-profile plate described in this Guide using reaction
volumes between 15µl and 50µl. Plates must be able to withstand the temperatures you are using
without any danger of deforming to the point where they fracture. Only low-profile plates should be
used. If standard height PCR plates are used, PrimeQ may be damaged and the warranty will be
invalid. Likewise if you put sections of cut-up plate in the block you must balance the plate sections
with empty sealed plate sections or previously used plate sections to balance the pressure on the
heated lid.
During the final cool-down, a ring of condensation may form on the inside walls of the plate wells,
above the liquid level but below the top of the sample block. This is not usually a cause for
concern as the condensation does not form during cycling, due to the action of the heated lid.