Techne PrimeQ - Manual (Page 3)

3
How to use this guide
Please read all the information in this guide before using the unit.
Le rogamos lea cuidadosamente la información contenida en este folleto antes de
manipular el aparato.
Veuillez lire attentivement toutes les instructions de ce document avant d’utiliser l’appareil.
This operator guide provides the basic information to get you started on the PrimeQ real-time PCR
system. It covers everything from taking the instrument out of the box, installing the application
software and system set-up, to setting up, running and analysing a range of real-time PCR
experiments. Background information is included at the start of each chapter to help familiarize the
user with the theory of operation and instrument setup.
This guide makes the assumption that the reader has a basic understanding of:
• Laboratory techniques; including assay design and preparation
• Microsoft Windows including terminology, common commands, file saving etc.
The guide is separated into the following main areas:
Chapter Title Content
Chapter 1 Safety and installation
information Instrument specifications
Warranty and certification
Site requirements; PC requirements
Installing Quansoft applications software
Filter installation
Chapter 2 Introduction to PrimeQ
and real-time PCR Overview of real-time PCR
Examples of fluorescent chemistries
PrimeQ system overview
Chapter 3 Using Quansoft Introduction to Quansoft
PCR program setup
Plate layout setup
Analysis setup
Starting/running/stopping/editing an experiment
Chapter 4 Data analysis Introduction/theory of data analysis
Quantification
Dissociation curve
Plus/minus scoring
Allelic discrimination
End-point analysis (Multi-read)
Multiplexing
Chapter 5 Technical information Maintenance and spare parts
Technical support