Techne PrimeQ - Manual (Page 74)

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• Calibrator (CAL): In relative quantification methods, the ratio of the DNA template in
different samples may be normalized to a calibrator sample. Particularly useful when
comparing Cq values (section 4.7).
• Reference Gene (REF): A common gene that is expressed in all samples at the same level.
Can be useful in relative quantification methods.
3.4.4.3 Choosing sample types
No Template Control, Standard and Unknown sample types are set as
defaults in Quansoft, represented by the icons displayed above the
plate layout.
Sample types: Use this function to choose and define different sample types. Click
once to bring up the Sample Types box (this can also be accessed from the menu bar
by clicking Tools/Define Sample Types…).
• To add a sample type to the Active list, simply highlight the sample type required from the
Available list and click the left arrow to move from the available into the active window. This
will then appear as an icon on the Plate Layout Editor main page next to the default icons
for standard (STD) and unknown (UNK).
• To remove a sample type, simply highlight the sample name in the active list and click on
the right-pointing arrow to transfer it from the active to the available list.
3.4.4.4 Adding a custom sample type
From the Sample Types box, click on Add Custom
and a settings box will appear.
For the custom sample type, choose the icon colour
and assign a name and abbreviation. Click OK when
done.
The custom sample type will now appear in the
available list. Move over to the active list using the
arrow if the sample type is to be used in the current
experiment. Close the sample types setting box and
return to the Plate Layout main screen.