Olympus IX81 - Manual (Page 40)

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Fig. 49
Fig. 50
Fig. 51
5 Cross-Nicol Adjustment (Figs. 49 to 51)
1. Rotate the condenser’s turret for the BF (brightfield) light path (with no
optical element engaged in the light path).
2. Move the polarizer detaching lever 1 on the IX-LWPO polarizer to engage
the polarizer in the light path. (Fig. 49)
3. Engage the 10X objective in the light path, place an optimum specimen for
brightfield observation on the stage, bring the specimen into approximate
and remove the specimen out of the light path.
4. When the U-BI90CT, binocular observation tube is used, rotate the CT
turret 2 to position “CT” to engage the CT lens in the light path. (Fig. 50)
When the U-BI90/U-TBI90 binocular observation tube or U-TR30H-2
trinocular observation tube is used, remove an eyepiece and attach
the U-CT30 centering telescope.
5. Rotate the focusing ring 3 (or, with the centering telescope, rotate the
knurled section) to bring the objective’s exit pupil into focus. (Fig. 50)
6. Move the prism movement knob 7 of the DIC slider in the clockwise
direction around the axis until, the knob is stopped. A black interference
stripe then a rainbow-colored interference stripe will be observed. Here,
stop the knob at the position with which the black interference stripe can
be seen. (Figs. 46 & 51)
7. While observing the objective’s exit pupil, rotate the polarizer rotation/
clamping knob 4 on the polarizer unit horizontally until the black interfer-
ence stripe becomes darkest. This is the position for use of the polarizer.
(Figs. 49 & 51)
8. After determining the position, clamp the polarizer rotation/clamping knob
so that the polarizer will not rotate.
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