Olympus IX81 - Manual (Page 41)

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IX81
6 Observation Method
1. Rotate the condenser turret to engage the suitable optical element for
the objective in use in the light path.
2. Engage the objective to be used in the light path.
3. Place the specimen on the stage and bring the specimen into focus by
moving the objective up or down.
4. Adjust the field iris diaphragm so that its image circumscribes the field of
view.
5. Adjust the aperture iris diaphragm to enhance the contrast.
6. Move the prism movement knob of the DIC slider to select the interfer-
ence color that can provide the optimum contrast in accordance with the
specimen.
U-DICT:
The background interference color is continuously variable
from the gray sensitive color to purple sensitive color.
U-DICTS:
U-DICTHC:
U-DICTHR:
}With sensitive color observation using the U-UCD8, engage the U-
UCDTP530 1 plate (sensitive color plate) in the light path.
· Setting the background color to dark enables an observation like darkfield
observation.
· Setting the background color to gray provides observation with high con-
trast and 3D feeling with the gray sensitive color with which the sensitivity
is highest.
· Setting the background color to gray allows very small change in phase
to be observed as a change in color.
· There is a directional characteristic with the detection sensitivity because of
the configuration of the DIC prism. As a result, the contrast may sometimes
be improved by rotating the specimen on the stage.
# For simultaneous observation with reflected fluorescence observa-
tion, refer to the instruction manual entitled “Manual/Motorized Re-
flected Fluorescence System”.
The background interference color is continuously variable
from black to light gray.