Olympus IX81 - Manual (Page 42)

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Fig. 52
5-3 Simplified Polarized Light Observation
}An analyzer and polarizer are required for simplified polarized light observation. The applicable condenser is the
IX2-LWUCDA2 or IX2-LWUCD. Simplified polarized light observation is not available with the IX-ULWCD.
1 Attaching the Analyzer and Polarizer
}Use the same procedure as that for attaching the analyzer and polarizer
for DIC observation (see page 34).
2 Observation Method (Fig. 52)
1. Rotate the turret for the BF (brightfield) light path (with no optical element
engaged in the light path).
2. Move the polarizer detaching lever 1 on the IX-LWPO polarizer unit to
engage the polarizer in the light path. (Fig. 52)
3. Attach the objective to be used to the revolving nosepiece and rotate it to
engage the objective in the light path.
4. Move the polarizer rotation/clamping knob 2 on the polarizer unit hori-
zontally in the counterclockwise direction around the axis until the posi-
tion with which the field of view is darkest. (Fig. 52)
5. Clamp the polarizer rotation/clamping knob so that the polarizer will not
rotate.
6. Place a specimen on the stage and bring the specimen into focus. Now
the specimen can be observed with simplified polarized light observa-
tion.
7. Adjust the field iris diaphragm so that its image circumscribes the field of
view.
8. Adjust the aperture iris diaphragm to enhance the contrast.
5-4 Reflected Light Fluorescence Observation (Separate Manual)
}Refer to the separate instruction manual.
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