Nitrogen (total), Rn035, 00 nitrogen (total) – YSI pHotoFlex Series User Manual

Page 49

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03/2013

Note: Before using the test with your photometer for the first time, determine the reagent blank
value.

YSI model no.:

14537

Category:

KT (reaction cell test)

Cell:

16 mm

Measuring range:

0.50 - 15.00 mg/l N

Pipette 10.0 ml of
sample into an empty
cell.

Add 1 level blue
microspoon of N-1K.

Add 6 drops of N-2K,
close cell with screw cap
and mix.

Heat the cell in the
thermoreactor for one
hour at 120 °C.

Place the cell in the cell
rack again and let it cool
down to room
temperature (=prepared
sample
).

Add 1 level blue
microspoon of N-3K into
a reaction cell and close
the cell with the screw
cap.

To dissolve solids, shake
the cell vigorously for 1
minute
.

With a pipette add 1.5 ml
of prepared sample very
slowly, close with screw
cap and mix vigorously.
Caution, cell becomes
very hot!

Allow to react for 10
minutes.

Insert the cell in the
photometer cell shaft
and start measurement.

Notes:

We recommend to determine a new reagent blank value (deionized water instead of sample) for each test package started.

Sample solution and reagents must have a temperature of 20-25 °C. Temper as necessary.

For further notes please refer to the package insert of the test.

10:00

Nitrogen (total)

Program no.

35

*RN035*

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