Batch commands and procedures – Luminex 100 IS Version 2.2 User Manual

Page 109

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MAP Technology

Using Luminex 100 IS 2.2 Software

PN 89-00002-00-069 Rev. A

6 - 55

The assay kit manufacturer may provide templates in the kits which
they distribute on floppy disk or CD ROM. Templates typically
include assay standards, controls, and maintenance commands (such
as washes or primes to acquire along with samples). OEM
manufacturers may provide templates preinstalled with your system.

The kit manufacturer includes assay reagents in the assay kit. You
must provide information about these reagents, such as lot numbers
and concentration values for the standards and assay controls.

A batch can include samples across more than one plate. When
setting up a batch, if the number of samples exceeds the wells in one
microtiter plate another plate appears for additional samples. The
new plate appears to the immediate right of the existing plate image
on the screen with a dark line between the adjacent columns of the
two plates.

During acquisition, as the system processes samples, the Run Batch
tab displays the wells containing the samples in the microtiter plate.
Colors indicate the system progress in analyzing the samples. The
following well colors indicate well-acquisition states:

Green well:

with command number—sample not
acquired.

Yellow well:

sample currently in acquisition

Red well:

sample failed. Check the system monitor for
more information

Green background: with check mark—successfully completed

The System Monitor (on Diagnostic tab) also indicates the well
acquisition status and highlights the current well in progress.

Batch Commands and
Procedures

Create a New

Batch

‹

To create a new batch:

1. Read the instructions that are provided with the assay kit you are

using. Follow the instructions for any preparations.

Note:

Common sample

errors are due to lower
number of events acquired
then established in the
template.

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