Jenway 6285 User Manual

Page 25

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Section 8

Calibration

1. Before beginning the calibration, ensure that close to hand, are the

appropriate cuvette/s, blank solution and standard solutions (as set in the
CALIBRATION SETUP

of the MEASURE SETUP menu).

2. Using the steps as detailed in the 'Using a stored method' section,

select the required method.

3. Once in measure mode, press the CAL key once to initiate the calibration

sequence.

4. The instrument display will show the message "Please insert blank…"










5. Taking care not to mark the optical surfaces, place the cuvette containing

the blank solution in the cuvette holder of the sample chamber and close
the lid.

6. Measure the fluorescence of the blank by pressing either the CAL key or

with the CAL option highlighted on the instrument screen. Press the enter
key to confirm.

7. The instrument screen will show the status message "Reading…" for the

duration of the measurement.










8. Once complete, the concentration (zero, as this is the blank) will be

shown as the large number on screen. Below this are the raw
fluorescence and the percentages of full-scale emission for the signal and
reference.










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