Jenway 6285 - Manual (Page 6)

10. Check the linear range for each method and, where necessary, use a multi-point
calibration or calibration curve.
11. Be aware of the effects of quenching and, where necessary, use sample dilution
or extraction methods to eliminate this.
12. Monitor the blank during and between batches to identify any increase due to
contamination.
13. Sources of contamination to be considered include cleaning agents, micro-
organisms, particles in suspension, stop-cock grease, filter paper residues and
plasticisers leached from containers, caps or sealing materials.
14. Many fluorescent assays are pH dependent. Ensure the pH of all samples and
standards is within specified limits before carrying out the analysis.
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