Jenway 6285 - Manual (Page 26)

9. The options at the bottom of the screen will have also changed. The
ACCEPT and REPEAT options relate to the calibration step just
completed. If the concentration and other data from the calibration are
correct and satisfactory, select ACCEPT to continue through the
calibration sequence to the first standard. If the previous step has given
unacceptable figures, then select REPEAT and perform the previous step
again. ABORT allows the whole calibration sequence to be cancelled and
the instrument to return to the measure screen.
10. Once the blank calibration has been accepted, the instrument will display
the message "Please insert standard x.xxx" and the concentration of
the first standard, as set in the STANDARDS TABLE of the
MEASURE/CALIBRATION SETUP
.
11. Remove the cuvette of Blank solution from the sample chamber, replace
with a cuvette of the requested standard and close the lid. If using
reusable cuvettes, ensure that proper cleaning procedures are employed
to make certain that there is no carry over of solutions or contamination.
12. As with the blank, calibrate the instrument with this first standard by using
the CAL option on screen or CAL key.
13. As with the blank, the concentration of the standard (as set in the
STANDARDS TABLE ) will be shown on screen with the raw
fluorescence and the percentages of full-scale emission for the signal and
reference.
14. Once again, review this data and if acceptable use the ACCEPT option to
move on to any further calibration standards in the same way. If not the
REPEAT
function can be used.
15. The sequence of calibrating with each standard will continue in this
fashion (points 10-13) until each standard that has been set, has been
used. At this point the instrument will give a summary of the calibration by
displaying the raw fluorescence for each standard in a table. An option to
view the calibration curve, a plot of the concentration of the standards
against their raw fluorescence, is given at the bottom of the screen (VIEW
CURVE
).
16. On exiting the calibration summary the instrument is returned to the
measure screen and the status message "Cal required…" will have been
removed.
17. Measurements of unknown concentration samples can now be made.
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