C.B.S. Scientific DGGEK-2401 User Manual

Page 19

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3 2 Gel Casting Techniques- continued

C Vertical gradient gel casting using gradient maker (GM-40) and a mini-

pump

1. Alternatively, you may choose to use a “mini-pump” or other peristaltic

pump to cast gels as shown in fig (3.6). If so, secure the gradient maker to

a ring stand and connect the outlet tubing to the mini-pump tubing adapter.

Connect tubing from mini-pump to a 20ga. needle for affixing between glass

plates.

Fig 3-6

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